Description
Description: This assay employs a two-site sandwich ELISA to quantitate ZFP36 in samples. An antibody specific for ZFP36 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZFP36 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFP36 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFP36 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The mouse tristetraprolin protein (TTP) is a basic proline-rich protein of molecular mass 33,600 Da that contains 3 PPPP repeats. Its mRNA level increases dramatically after stimulation with various mitogens. Also called ZFP36, Nup475, and TISII or TIS11, it has been localized to the cell nucleus and is thought to contain an unusual zinc finger structure.TTP, which is 87% identical to the mouse protein; the putative zinc finger structure is conserved among human, mouse and rat.C-terminal truncated forms of TTP, as well as a 77-amino acid fragment that contains both zinc fingers, could bind to the TNF-alpha ARE in cell-free crosslinking and gel shift assays. In addition, the truncated forms of TTP could stimulate the deadenylation and/or breakdown of TNF-alpha mRNA in intact cells